Device

Part:BBa_K2548000:Design

Designed by: Zhuoyi Yang   Group: iGEM18_BNDS_CHINA   (2018-08-30)


constitutive promoter-rbs-rhlR-terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 299
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 774


Design Notes

The constitutive promoter is BBa_J23100; the RBS sequence is BBa_B0034; rhlR (LVA tagged) is BBa_C0071; the random spacer is from the plasmid pET28a; the terminator is BBa_B0015.


Source

The rhlR sequence comes from P. aeruginosa PA3477 with LVA tag. rhlR protein acts as transcriptional regulator and binds with N-butyryl-HSL (C4-HSL).

References